Dose–response

Fit 3PL and 4PL dose-response curves for individual experiments. Estimate EC50 or IC50, inspect fit diagnostics and export scientific figures locally.

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ABOUT THIS ANALYSIS

Dose-response curve fitting: 3PL, 4PL, EC50 and IC50

Fit 3PL and 4PL dose-response curves for individual experiments. Estimate EC50 or IC50, inspect fit diagnostics and export scientific figures locally.

When to use it

Monotonic activation or inhibition measured across concentrations within each biological experiment.

What to enter

Import concentration and response measurements with series and experiment identifiers. Declare concentration units, curve direction and fixed plateaus; technical repeats are averaged at each dose.

What you get

Per-experiment logistic fits, relative EC50 or IC50, molar p-potency, plateau and Hill estimates, covariance-based intervals when available, residuals and SVG or PNG figures.

Assumptions and limits

Fits use equal weighting of dose means. Intervals rely on local linearity and error assumptions; they do not estimate between-experiment uncertainty. Biphasic curves and pooled biological inference need other methods.

Calculations run locally; participant data stays in browser memory. Download your results or project before leaving. This is beta software, with independent statistical review pending.